THE AMINO-ACID-SEQUENCE OF RAT-LIVER NONSPECIFIC LIPID TRANSFER PROTEIN (STEROL CARRIER PROTEIN-2) IS PRESENT IN A HIGH-MOLECULAR-WEIGHT PROTEIN - EVIDENCE FROM CDNA ANALYSIS

被引:48
作者
OSSENDORP, BC
VANHEUSDEN, GPH
WIRTZ, KWA
机构
[1] Centre for Biomembranes and Lipid Enzymology, State University of Utrecht, 3584 CH Utrecht, Transitorium III
关键词
D O I
10.1016/0006-291X(90)92367-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The affinity-purified antibody against rat liver non-specific lipid transfer protein (nsL-TP; sterol carrier protein 2) was used to screen a λ-gt11 rat liver cDNA library. Positive cDNA clones were further identified by Southern blot analysis and sequenced. The largest cDNA clone consisted of 1851 bp starting at the 5′ end with an open reading frame of 1545 bp. The 369 bp located at the 3′ end of this open reading frame corresponded with the amino acid sequence of nsL-TP. © 1990.
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页码:631 / 636
页数:6
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