CLONING OF AN ERWINIA-HERBICOLA GENE NECESSARY FOR GLUCONIC ACID PRODUCTION AND ENHANCED MINERAL PHOSPHATE SOLUBILIZATION IN ESCHERICHIA-COLI HB101 - NUCLEOTIDE-SEQUENCE AND PROBABLE INVOLVEMENT IN BIOSYNTHESIS OF THE COENZYME PYRROLOQUINOLINE QUINONE

被引:110
作者
LIU, ST
LEE, LY
TAI, CY
HUNG, CH
CHANG, YS
WOLFRAM, JH
ROGERS, R
GOLDSTEIN, AH
机构
[1] CALIF STATE UNIV LOS ANGELES, DEPT BIOL, 5151 STATE UNIV, LOS ANGELES, CA 90032 USA
[2] CHANG GUNG MED COLL, DEPT MICROBIOL & IMMUNOL, KWEI SHAN TAOYUAN, TAIWAN
[3] IDAHO NATL ENGN LAB, BIOTECHNOL GRP, IDAHO FALLS, ID 83415 USA
关键词
D O I
10.1128/jb.174.18.5814-5819.1992
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Escherichia coli is capable of synthesizing the apo-glucose dehydrogenase enzyme (GDH) but not the cofactor pyrroloquinoline quinone (PQQ), which is essential for formation of the holoenzyme. Therefore, in the absence of exogenous PQQ, E. coli does not produce gluconic acid. Evidence is presented to show that the expression of an Erwinia herbicola gene in E. coli HB101(pMCG898) resulted in the production of gluconic acid, which, in turn, implied PQQ biosynthesis. Transposon mutagenesis showed that the essential gene or locus was within a 1.8-kb region of a 4.5-kb insert of the plasmid pMCG898. This 1.8-kb region contained only one apparent open reading frame. In this paper, we present the nucleotide sequence of this open reading frame, a 1,134-bp DNA fragment coding for a protein with an M(r) of 42,160. The deduced sequence of this protein had a high degree of homology with that of gene III (M(r), 43,600) of a PQQ synthase gene complex from Acinetobacter calcoaceticus previously identified by Goosen et al. (J. Bacteriol. 171:447-455, 1989). In minicell analysis, pMCG898 encoded a protein with an M(r) of 41,000. These data indicate that E. coli HB101(pMCG898) produced the GDH-PQQ holoenzyme, which, in turn, catalyzed the oxidation of glucose to gluconic acid in the periplasmic space. As a result of the gluconic acid production, E. coli HB101(pMCG898) showed an enhanced mineral phosphate-solubilizing phenotype due to acid dissolution of the hydroxyapatite substrate.
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页码:5814 / 5819
页数:6
相关论文
共 20 条
[1]   D-GLUCOSE DEHYDROGENASE OF GLUCONOBACTER-SUBOXYDANS - SOLUBILIZATION, PURIFICATION AND CHARACTERIZATION [J].
AMEYAMA, M ;
SHINAGAWA, E ;
MATSUSHITA, K ;
ADACHI, O .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1981, 45 (04) :851-861
[2]   PRODUCTION OF 2-KETO-L-GULONATE, AN INTERMEDIATE IN L-ASCORBATE SYNTHESIS, BY A GENETICALLY MODIFIED ERWINIA-HERBICOLA [J].
ANDERSON, S ;
MARKS, CB ;
LAZARUS, R ;
MILLER, J ;
STAFFORD, K ;
SEYMOUR, J ;
LIGHT, D ;
RASTETTER, W ;
ESTELL, D .
SCIENCE, 1985, 230 (4722) :144-149
[3]   MUTANTS OF ESCHERICHIA-COLI PRODUCING PYRROLOQUINOLINE QUINONE [J].
BIVILLE, F ;
TURLIN, E ;
GASSER, F .
JOURNAL OF GENERAL MICROBIOLOGY, 1991, 137 :1775-1782
[4]   A COMPLEMENTATION ANALYSIS OF RESTRICTION AND MODIFICATION OF DNA IN ESCHERICHIA COLI [J].
BOYER, HW ;
ROULLAND.D .
JOURNAL OF MOLECULAR BIOLOGY, 1969, 41 (03) :459-&
[5]   QUINOPROTEINS - ENZYMES CONTAINING THE QUINONOID COFACTOR PYRROLOQUINOLINE QUINONE, TOPAQUINONE OR TRYPTOPHAN-TRYPTOPHAN QUINONE [J].
DUINE, JA .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 200 (02) :271-284
[6]   GLUCOSE-DEHYDROGENASE FROM ACINETOBACTER-CALCOACETICUS - QUINOPROTEIN [J].
DUINE, JA ;
FRANK, J ;
VANZEELAND, JK .
FEBS LETTERS, 1979, 108 (02) :443-446
[7]   MOLECULAR-CLONING AND REGULATION OF A MINERAL PHOSPHATE SOLUBILIZING GENE FROM ERWINIA-HERBICOLA [J].
GOLDSTEIN, AH ;
LIU, ST .
BIO-TECHNOLOGY, 1987, 5 (01) :72-74
[8]  
Goldstein AH, 1986, AM J ALTERNATIVE AGR, V1, P57, DOI DOI 10.1017/S0889189300000886
[9]   ACINETOBACTER-CALCOACETICUS GENES INVOLVED IN BIOSYNTHESIS OF THE COENZYME PYRROLO-QUINOLINE-QUINONE - NUCLEOTIDE-SEQUENCE AND EXPRESSION IN ESCHERICHIA-COLI K-12 [J].
GOOSEN, N ;
HORSMAN, HPA ;
HUINEN, RGM ;
VANDEPUTTE, P .
JOURNAL OF BACTERIOLOGY, 1989, 171 (01) :447-455
[10]   GAMMA-DELTA SEQUENCE OF F IS AN INSERTION SEQUENCE [J].
GUYER, MS .
JOURNAL OF MOLECULAR BIOLOGY, 1978, 126 (03) :347-365