2 OPEN COMPLEXES AND A REQUIREMENT FOR MG2+ TO OPEN THE LAMBDA-P(R) TRANSCRIPTION START SITE

被引：93

作者：

SUH, WC

ROSS, W

RECORD, MT

机构：

[1] UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706

[2] UNIV WISCONSIN,DEPT BIOCHEM,MADISON,WI 53706

[3] UNIV WISCONSIN,DEPT BACTERIOL,MADISON,WI 53706

来源：

SCIENCE | 1993年 / 259卷 / 5093期

关键词：

D O I：

10.1126/science.8420002

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Potassium permanganate (KMnO4) footprinting in the absence and presence of magnesium (Mg2+) at the lambdaP(R) promoter identified two different open complexes with Escherichia coli Esigma70 RNA polymerase (designated RP(o1) and RP(o2). The single-stranded region in RP(o1), (formed in the absence of Mg2+) was at most 12 bases long, whereas that in RP(o2) (formed in the presence of Mg2+) spanned at least 14 bases. Only in RP(o2) did the single-stranded region extend to the start point of transcription (+1, +2). These results provide a structural basis for the requirement for uptake of Mg2+ in the formation of RP(o2) from RP(o1), as deduced from kinetic studies at this promoter.

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页码：358 / 361

页数：4

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