SYNTHESIS OF BARNASE SITE-SPECIFICALLY LABELED WITH 2 C-13 ATOMS USING PARTIALLY PROTECTED PEPTIDE THIOESTER BUILDING-BLOCKS

被引：16

作者：

HOJO, H ^{[1
]}

AIMOTO, S ^{[1
]}

机构：

[1] OSAKA UNIV, INST PROT RES, 3-2 YAMADAOKA, SUITA, OSAKA 565, JAPAN

来源：

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN | 1993年 / 66卷 / 10期

关键词：

D O I：

10.1246/bcsj.66.3004

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Barnase, site-specifically labelled with two C-13 atoms, was synthesized using partially protected peptide thioesters as building blocks. Four partially protected peptide segments (Boc-[Lys(Boc) 19,27 ]-barnase(1-34)-SC(CH3)2CH2CO-Nle-NH2, iNoc-[Lys(BoC)39,49 1-barnase(35-52)-SC(CH3)2CH2CO-Nle-NH2, iNoc-[Lys-(Boc)62,66]-barnase(53-81)-SC(CH3)2CH2CO-Nle-NH2, [Lys(Boc)98,108]-barnase(82-110)) were successively condensed in the presence of silver ions and N-hydroxysuccinimide (HONSu). Finally, barnase with full ribonuclease activity was obtained in a yield of 11% based on the carboxyl terminal peptide segment.

引用

页码：3004 / 3008

页数：5

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