TRANSFORMATION OF BACILLUS-SUBTILIS, ANTIFUNGAL-ANTIBIOTIC ITURIN PRODUCERS WITH ISOLATED ANTIBIOTIC-RESISTANCE PLASMIDS

被引：5

作者：

MATSUNO, Y ^{[1
]}

HITOMI, T ^{[1
]}

ANO, T ^{[1
]}

SHODA, M ^{[1
]}

机构：

[1] TOKYO INST TECHNOL, RESOURCES UTILIZAT RES LAB, 4259 NAGATSUTA, MIDORI KU, YOKOHAMA, KANAGAWA 227, JAPAN

来源：

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY | 1992年 / 38卷 / 01期

关键词：

D O I：

10.2323/jgam.38.13

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In order to obtain appropriate vectors to transform several strains of Bacillus subtilis, antifungal iturin producers, plasmids have been isolated as covalently closed circular deoxyribonucleic acid from antibiotic-resistant bacteria inhabited in composts. Eight plasmids coding antibiotic resistance transformed both competent cells of a derivative of B. subtilis Marburg 168 and protoplasts of B. subtilis NB22, an antifungal-antibiotic iturin producer, in the presence of polyethylene glycol. However, transformation efficiency was not high enough as a cloning host-vector system. To improve transformation efficiency, KCl-treatment method and electroporation method were applied to four iturin producers, and electroporation method was most effective for transformation with newly-isolated plasmids with great efficiency.

引用

页码：13 / 21

页数：9

共 24 条

[1] REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS [J].