SUBSTRATE UPTAKE MECHANISMS FOR YEAST-CELLS - NEW APPROACH UTILIZING A FLUOROMETER

被引：20

作者：

EINSELE, A ^{[1
]}

RISTROPH, DL ^{[1
]}

HUMPHREY, AE ^{[1
]}

机构：

[1] UNIV PENN,SCH CHEM ENGN,PHILADELPHIA,PA 19104

来源：

EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1979年 / 6卷 / 04期

关键词：

D O I：

10.1007/BF00499163

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This paper reports on experiments in which a fluorometer, which permits culture fluorescence to be measured in situ was coupled to a bioreactor. The fluorometer through external light excitement NADH can be made to fluoresce, thus allowing intracellular NADH profiles to be measured in response to extracellular step-changes in substrate concentration. Experiments with baker's yeast and Candida tropicalis as organisms and glucose and hexadecane as substrates are reported. The results suggest different uptake mechanisms depending on cell type and on substrate. © 1979 Springer-Verlag.

引用

页码：335 / 339

页数：5

共 6 条

[1] MIXING TIMES AND GLUCOSE-UPTAKE MEASURED WITH A FLUOROMETER
EINSELE, A
RISTROPH, DL
HUMPHREY, AE
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 1978, 20 (09) : 1487 - 1492
[2] Harrison D E, 1976, Adv Microb Physiol, V14, P243, DOI 10.1016/S0065-2911(08)60229-5
[3] Kappell O, 1976, BIOTECHNOL BIOENG, V18, P967
[4] Kotyk A., 1975, CELL MEMBRANE TRANSP
[5] WATTENEUW CM, BIOTECHNOL BIOENG S
[6] ESTIMATION OF FERMENTATION BIOMASS CONCENTRATION BY MEASURING CULTURE FLUORESCENCE
ZABRISKIE, DW
HUMPHREY, AE
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1978, 35 (02) : 337 - 343

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