CONALBUMIN-CONJUGATED SILICA-GEL, A NEW CHIRAL STATIONARY PHASE FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：29

作者：

MANO, N ^{[1
]}

ODA, Y ^{[1
]}

MIWA, T ^{[1
]}

ASAKAWA, N ^{[1
]}

YOSHIDA, Y ^{[1
]}

SATO, T ^{[1
]}

机构：

[1] EISAI & CO LTD,DEPT PHARMACEUT RES LAB,KAWASHIMA,GIFU 483,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 603卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)85350-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new chiral stationary phase using conalbumin (from chicken egg white) was developed for high-performance liquid chromatography. Chiral resolution of racemic azelastine, an antiallergic drug, was achieved on a conalbumin-conjugated silica gel column. The effects of the pH, the concentration of organic solvents and salts in the mobile phase, and the temperature on the capacity factor and resolution of racemic azelastine were examined. This column shows good stability and can separate optical isomers with an aqueous mobile phase. It should be very useful in studies on pharmacokinetics and in clinical chemistry.

引用

页码：105 / 109

页数：5

共 15 条

[1] DIRECT LIQUID-CHROMATOGRAPHIC SEPARATION OF ENANTIOMERS ON IMMOBILIZED PROTEIN STATIONARY PHASES .3. OPTICAL RESOLUTION OF A SERIES OF N-AROYL D,L-AMINO ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON BOVINE SERUM-ALBUMIN COVALENTLY BOUND TO SILICA [J].

ALLENMARK, S ;

BOMGREN, B ;

BOREN, H .

JOURNAL OF CHROMATOGRAPHY, 1983, 264 (01) :63-68

[2] INHIBITION OF CALCIUM IONOPHORE (A23187)-STIMULATED HISTAMINE-RELEASE FROM RAT PERITONEAL MAST-CELLS BY AZELASTINE - IMPLICATIONS FOR ITS MODE OF ACTION [J].

CHAND, N ;

PILLAR, J ;

DIAMANTIS, W ;

PERHACH, JL ;

SOFIA, RD .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1983, 96 (3-4) :227-233

[3] DIRECT LIQUID-CHROMATOGRAPHIC RESOLUTION OF RACEMIC DRUGS USING ALPHA-1-ACID GLYCOPROTEIN AS THE CHIRAL STATIONARY PHASE [J].

HERMANSSON, J .

JOURNAL OF CHROMATOGRAPHY, 1983, 269 (02) :71-80

[4] THE EFFECTS OF PH AND ALCOHOLIC ORGANIC MODIFIERS ON THE DIRECT SEPARATION OF SOME ACIDIC, BASIC AND NEUTRAL COMPOUNDS ON A COMMERCIALLY AVAILABLE OVOMUCOID COLUMN [J].

IREDALE, J ;

AUBRY, AF ;

WAINER, I .

CHROMATOGRAPHIA, 1991, 31 (7-8) :329-334

[5] OPTICAL RESOLUTION BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY .1. SEPARATION OF ENANTIOMERIC AZERASTINE HYDROCHLORIDE BY ION-PAIR CHROMATOGRAPHY [J].

KAJIMA, T ;

ASAKAWA, N ;

HATTORI, T ;

SATO, T ;

MIYAKE, Y .

YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 1989, 109 (08) :570-573

[6] COMPARISON OF A NEW OVOMUCOID AND A 2ND-GENERATION ALPHA-1-ACID GLYCOPROTEIN-BASED CHIRAL COLUMN FOR THE DIRECT HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY RESOLUTION OF DRUG ENANTIOMERS [J].

KIRKLAND, KM ;

NEILSON, KL ;

MCCOMBS, DA .

JOURNAL OF CHROMATOGRAPHY, 1991, 545 (01) :43-58

[7]

LITTLE MM, 1987, J ALLERGY CLIN IMMUN, V79, P204

[8] APPLICATION OF AN OVOMUCOID-CONJUGATED COLUMN FOR THE OPTICAL RESOLUTION OF SOME PHARMACEUTICALLY IMPORTANT COMPOUNDS [J].

MIWA, T ;

MIYAKAWA, T ;

KAYANO, M ;

MIYAKE, Y .

JOURNAL OF CHROMATOGRAPHY, 1987, 408 :316-322

[9]

MIWA T, 1987, CHEM PHARM BULL, V35, P682

[10] CHARACTERISTICS OF OVOMUCOID-CONJUGATED COLUMNS IN THE DIRECT LIQUID-CHROMATOGRAPHIC RESOLUTION OF RACEMIC COMPOUNDS [J].

MIWA, T ;

KURODA, H ;

SAKASHITA, S ;

ASAKAWA, N ;

MIYAKE, Y .

JOURNAL OF CHROMATOGRAPHY, 1990, 511 :89-95

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