SERUM BIOACTIVE FOLLICLE-STIMULATING HORMONE-LIKE ACTIVITY IN HUMAN-PREGNANCY IS A METHODOLOGICAL ARTIFACT

被引:19
作者
SIMONI, M
KHAN, SA
NIESCHLAG, E
机构
[1] UNIV MUNSTER, INST REPROD MED, STEINFURTER STR 107, W-4400 MUNSTER, GERMANY
[2] WHO, COLLABORATING CTR RES HUMAN REPROD, W-4400 MUNSTER, GERMANY
关键词
D O I
10.1210/jcem-73-5-1118
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Serum from pregnant women has been shown to contain both FSH-like and FSH antagonistic activities when measured by an in vitro bioassay based upon FSH-dependent aromatase activity of immature rat Sertoli cells. In the present study we further tested the hypothesis that the FSH-like bioactivity of pregnancy serum was due to an authentic aromatase stimulator. A potent inhibitor of aromatase which completely blocked the FSH action on Sertoli cells had no effect on the bioactivity of pregnancy serum. Experiments using conversion of tritiated testosterone to estradiol showed that the factor did not stimulate aromatase activity in rat Sertoli cells. After incubation with pregnancy serum, equally high amounts of estradiol were measurable in the medium in both the absence and presence of Sertoli cells. The activity was almost completely lost after charcoal treatment or ether extraction of the serum and was shown to probably be due to the release of endogenous estrogens by the carrier proteins in the incubation medium of the Sertoli cell assay. These data suggest that the FSH-like bioactivity in serum from pregnant women is an artifact due to nonspecific interference(s) in the bioassay.
引用
收藏
页码:1118 / 1122
页数:5
相关论文
共 17 条
  • [11] QUALITATIVE CHANGES IN URINARY GONADOTROPHINS IN HUMAN PREGNANCY DURING THE PERIOD OF RAPID INCREASE IN HORMONE TITER
    LYON, RA
    SIMPSON, ME
    EVANS, HM
    [J]. ENDOCRINOLOGY, 1953, 53 (06) : 674 - 686
  • [12] SERUM BIOACTIVE FOLLICLE-STIMULATING HORMONE-LIKE ACTIVITY INCREASES DURING PREGNANCY
    PADMANABHAN, V
    SONSTEIN, J
    OLTON, PL
    NIPPOLDT, T
    MENON, KMJ
    MARSHALL, JC
    KELCH, RP
    BEITINS, IZ
    [J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1989, 69 (05) : 968 - 977
  • [13] AN IMPROVED INVITRO BIOASSAY FOR FOLLICLE-STIMULATING-HORMONE (FSH) - SUITABLE FOR MEASUREMENT OF FSH IN UNEXTRACTED HUMAN-SERUM
    PADMANABHAN, V
    CHAPPEL, SC
    BEITINS, IZ
    [J]. ENDOCRINOLOGY, 1987, 121 (03) : 1089 - 1098
  • [14] MODULATION OF SERUM FOLLICLE-STIMULATING-HORMONE BIOACTIVITY AND ISOFORM DISTRIBUTION BY ESTROGENIC STEROIDS IN NORMAL WOMEN AND IN GONADAL-DYSGENESIS
    PADMANABHAN, V
    LANG, LL
    SONSTEIN, J
    KELCH, RP
    BEITINS, IZ
    [J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1988, 67 (03) : 465 - 473
  • [15] SIITERI PK, 1982, RECENT PROG HORM RES, V38, P457
  • [16] STIMULATORY AND INHIBITORY INFLUENCES OF SERUM FROM PREGNANT-WOMEN ON AROMATASE-ACTIVITY OF IMMATURE RAT SERTOLI CELLS
    SIMONI, M
    KHAN, SA
    DEGEYTER, C
    NIESCHLAG, E
    [J]. ACTA ENDOCRINOLOGICA, 1989, 121 (02): : 265 - 269
  • [17] SENSITIVE AND SPECIFIC INVITRO BIOASSAY METHOD FOR THE MEASUREMENT OF FOLLICLE-STIMULATING-HORMONE ACTIVITY
    VANDAMME, MP
    ROBERTSON, DM
    MARANA, R
    RITZEN, EM
    DICZFALUSY, E
    [J]. ACTA ENDOCRINOLOGICA, 1979, 91 (02): : 224 - 237