INDUCED REDISTRIBUTION OF CELL-SURFACE RECEPTORS BY ALTERNATING-CURRENT ELECTRIC-FIELDS

被引:45
作者
CHO, MR
THATTE, HS
LEE, RC
GOLAN, DE
机构
[1] HARVARD UNIV,SCH MED,DEPT BIOL CHEM & MOLEC PHARMACOL,BOSTON,MA 02115
[2] HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02115
[3] BRIGHAM & WOMENS HOSP,DIV HEMATOL ONCOL,BOSTON,MA 02115
[4] HARVARD UNIV,SCH MED,DEPT RADIOL,BOSTON,MA 02115
[5] BETH ISRAEL HOSP,BOSTON,MA 02215
[6] UNIV CHICAGO,DEPT SURG,CHICAGO,IL 60637
[7] UNIV CHICAGO,DEPT ORGANISMAL BIOL,CHICAGO,IL 60637
关键词
CELLULAR ANISOTROPY; TRANSFERRIN RECEPTORS; LOW DENSITY; LIPOPROTEIN RECEPTORS; AC ELECTRIC FIELD;
D O I
10.1096/fasebj.8.10.8050677
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The molecular mechanisms that underlie the biological effects of low frequency sinusoidal electric fields may involve induced changes in the physical state of charged cell surface receptors. We have used intensified fluorescence video microscopy to study the redistribution of cell surface receptors, including transferrin receptors (T(F)R) and low density lipoprotein receptors (LDL-R), in response to externally applied alternating current electric fields in the 3 to 23 V/cm range (peak to peak). Redistribution of both T(F)R and LDL-R was prominent at frequencies of 1 and 10 Hz but negligible at frequencies of 60 and 120 Hz. Application of a 1 Hz, 23 V/cm field for 15 min caused a twofold change in local T(F)R surface density, whereas application of a 60 Hz, 23 V/cm field resulted in no significant T(F)R redistribution. The extent of T(F)R redistribution induced by a 1 Hz field changed by only 20% over the field strength range from 3.5 to 23 V/cm. AC field-induced cell surface receptor migration did not consistently follow electric field lines, suggesting that mechanisms more complex than classical electrophoresis and electroosmosis mediate receptor redistribution. Joule heating and plasma membrane calcium channel activation were shown not to be involved in the mechanism of receptor redistribution. Applied external electric fields may reorganize cytoskeletal and plasma membrane structures, providing pathways for cell surface receptors to migrate anharmonically.
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页码:771 / 776
页数:6
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