SELECTIVE PURIFICATION OF MICROTUBULE-ASSOCIATED PROTEIN-1 AND PROTEIN-2 FROM RAT-BRAIN USING POLY(L-ASPARTIC ACID)

被引：14

作者：

FUJII, T ^{[1
]}

NAKAMURA, A ^{[1
]}

OGOMA, Y ^{[1
]}

KONDO, Y ^{[1
]}

ARAI, T ^{[1
]}

机构：

[1] UNIV TSUKUBA,INST BASIC MED SCI,TSUKUBA,IBARAKI 305,JAPAN

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 184卷 / 02期

关键词：

Amino acids - Ionic strength - Purification - Proteins;

D O I：

10.1016/0003-2697(90)90679-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and selective purification procedure for microtubule-associated protein (MAP) 1 and MAP 2 has been established. This procedure is based upon the fact that poly(l-aspartic acid) (PLAA) can specifically remove MAP 1 from microtubules polymerized by taxol (Nakamura et al., 1989, J. Biochem. 106, 93-97). MAP 1 released by PLAA was further purified by column chromatography on phosphocellulose and Bio-Gel A-15m. The purified MAP 1 contained MAPs 1A and 1B. From microtubules devoid of MAP 1, MAP 2, consisting of MAPs 2A and 2B, could also be isolated by exposure to high ionic strength solutions in the presence of taxol without heat treatment. Both MAPs 1 and 2 cosedimented with microtubules consisting of purified tubulin. © 1990.

引用

页码：268 / 273

页数：6

共 31 条

[21] MICROTUBULE ASSEMBLY IN ABSENCE OF ADDED NUCLEOTIDES [J].

SHELANSK.ML ;

GASKIN, F ;

CANTOR, CR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (03) :765-768

[22]

SHIOMURA Y, 1987, J NEUROSCI, V7, P1461

[23] CYCLIC AMP-DEPENDENT ENDOGENOUS PHOSPHORYLATION OF A MICROTUBULE-ASSOCIATED PROTEIN [J].

SLOBODA, RD ;

RUDOLPH, SA ;

ROSENBAUM, JL ;

GREENGARD, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (01) :177-181

[24] MICROTUBULE-ASSOCIATED PROTEINS AND STIMULATION OF TUBULIN ASSEMBLY INVITRO [J].

SLOBODA, RD ;

DENTLER, WL ;

ROSENBAUM, JL .

BIOCHEMISTRY, 1976, 15 (20) :4497-4505

[25] ELECTROPHORETIC TRANSFER OF PROTEINS FROM POLYACRYLAMIDE GELS TO NITROCELLULOSE SHEETS - PROCEDURE AND SOME APPLICATIONS [J].

TOWBIN, H ;

STAEHELIN, T ;

GORDON, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (09) :4350-4354

[26] LOW-MOLECULAR WEIGHT MICROTUBULE-ASSOCIATED PROTEINS ARE LIGHT-CHAINS OF MICROTUBULE-ASSOCIATED PROTEIN-1 (MAP-1) [J].

VALLEE, RB ;

DAVIS, SE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (05) :1342-1346

[27] A TAXOL-DEPENDENT PROCEDURE FOR THE ISOLATION OF MICROTUBULES AND MICROTUBULE-ASSOCIATED PROTEINS (MAPS) [J].

VALLEE, RB .

JOURNAL OF CELL BIOLOGY, 1982, 92 (02) :435-442

[28]

VALLEE RB, 1986, ANN NY ACAD SCI, V466, P133

[29] HIGH-MOLECULAR-WEIGHT MICROTUBULE ASSOCIATED PROTEINS (MAPS) - A UBIQUITOUS FAMILY OF CYTOSKELETAL CONNECTING LINKS [J].

WICHE, G .

TRENDS IN BIOCHEMICAL SCIENCES, 1985, 10 (02) :67-70

[30] CA-2', CALMODULIN-DEPENDENT REGULATION OF MICROTUBULE FORMATION VIA PHOSPHORYLATION OF MICROTUBULE-ASSOCIATED PROTEIN-2, TAU-FACTOR, AND TUBULIN, AND COMPARISON WITH THE CYCLIC AMP-DEPENDENT PHOSPHORYLATION [J].

YAMAMOTO, H ;

FUKUNAGA, K ;

GOTO, S ;

TANAKA, E ;

MIYAMOTO, E .

JOURNAL OF NEUROCHEMISTRY, 1985, 44 (03) :759-768

← 1 2 3 4 →