PRELIMINARY CRYSTALLOGRAPHIC STUDIES OF UREASE FROM JACK BEAN AND FROM KLEBSIELLA-AEROGENES

被引：49

作者：

JABRI, E

LEE, MH

HAUSINGER, RP

KARPLUS, PA

机构：

[1] MICHIGAN STATE UNIV,DEPT MICROBIOL,E LANSING,MI 48824

[2] CORNELL UNIV,BIOCHEM MOLEC & CELL BIOL SECT,ITHACA,NY 14853

[3] MICHIGAN STATE UNIV,DEPT BIOCHEM,E LANSING,MI 48824

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 227卷 / 03期

关键词：

UREASE; NICKEL; PROTEIN CRYSTALLOGRAPHY;

D O I：

10.1016/0022-2836(92)90232-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ureases from both jack bean (Canavalia ensiformis) seeds and Klebsiella aerogenes have been crystallized by the hanging drop method. The plant-derived urease crystals are regular octahedra analogous to those obtained by Sumner. Preliminary X-ray diffraction studies show that the crystals belong to the cubic space group F4132, with a = 364 A ̊, and appear to contain one or two subunits in the asymmetric unit. Using a synchrotron source, the crystals diffract to near 3·5 Å resolution. Crystals of urease from K. aerogenes belong to the cubic space group I23 or I213, with a = 170·8 A ̊ and appear to contain a single catalytic unit per asymmetric unit. The crystals diffract to better than 2·0 Å resolution and are well suited for structural analysis. © 1992.

引用

收藏

页码：934 / 937

页数：4

相关论文

共 19 条

[1] JACK BEAN UREASE (EC3.5.1.5) . A NEW PURIFICATION AND RELIABLE RATE ASSAY [J].

BLAKELEY, RL ;

WEBB, EC ;

ZERNER, B .

BIOCHEMISTRY, 1969, 8 (05) :1984-&

[2] X-RAY ABSORPTION SPECTROSCOPIC EVIDENCE FOR BINDING OF THE COMPETITIVE INHIBITOR 2-MERCAPTOETHANOL TO THE NICKEL SITES OF JACK BEAN UREASE - A NEW NI-NI INTERACTION IN THE INHIBITED ENZYME [J].

CLARK, PA ;

WILCOX, DE ;

SCOTT, RA .

INORGANIC CHEMISTRY, 1990, 29 (04) :579-581

[3] JACK BEAN UREASE (EC-3515) .4. THE MOLECULAR-SIZE AND THE MECHANISM OF INHIBITION BY HYDROXAMIC ACIDS - SPECTROPHOTOMETRIC TITRATION OF ENZYMES WITH REVERSIBLE INHIBITORS [J].

DIXON, NE ;

HINDS, JA ;

FIHELLY, AK ;

GAZZOLA, C ;

WINZOR, DJ ;

BLAKELEY, RL ;

ZERNER, B .

CANADIAN JOURNAL OF BIOCHEMISTRY, 1980, 58 (12) :1323-1334

[4] JACK BEAN UREASE (EC 3.5.1.5) .2. THE RELATIONSHIP BETWEEN NICKEL, ENZYMATIC-ACTIVITY, AND THE ABNORMAL ULTRAVIOLET-SPECTRUM - THE NICKEL CONTENT OF JACK BEANS [J].

DIXON, NE ;

GAZZOLA, C ;

ASHER, CJ ;

LEE, DSW ;

BLAKELEY, RL ;

ZERNER, B .

CANADIAN JOURNAL OF BIOCHEMISTRY, 1980, 58 (06) :474-480

[5] JACK-BEAN UREASE (EC 3.5.1.5) - METALLOENZYME - SIMPLE BIOLOGICAL ROLE FOR NICKEL [J].

DIXON, NE ;

GAZZOLA, C ;

BLAKELEY, RL ;

ZERNER, B .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1975, 97 (14) :4131-4133

[6] JACK BEAN UREASE (EC-3515) .5. ON THE MECHANISM OF ACTION OF UREASE ON UREA, FORMAMIDE, ACETAMIDE, N-METHYLUREA, AND RELATED-COMPOUNDS [J].

DIXON, NE ;

RIDDLES, PW ;

GAZZOLA, C ;

BLAKELEY, RL ;

ZERNER, B .

CANADIAN JOURNAL OF BIOCHEMISTRY, 1980, 58 (12) :1335-1344

[7] VARIABLE-TEMPERATURE MAGNETIC CIRCULAR-DICHROISM SPECTROSCOPY AS A PROBE OF THE ELECTRONIC AND MAGNETIC-PROPERTIES OF NICKEL IN JACK BEAN UREASE [J].

FINNEGAN, MG ;

KOWAL, AT ;

WERTH, MT ;

CLARK, PA ;

WILCOX, DE ;

JOHNSON, MK .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (10) :4030-4032

[8] SPARSE-MATRIX SAMPLING - A SCREENING METHOD FOR CRYSTALLIZATION OF PROTEINS [J].

JANCARIK, J ;

KIM, SH .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1991, 24 :409-411

[9] SOLVENT CONTENT OF PROTEIN CRYSTALS [J].

MATTHEWS, BW .

JOURNAL OF MOLECULAR BIOLOGY, 1968, 33 (02) :491-+

[10] MICROBIAL UREASES - SIGNIFICANCE, REGULATION, AND MOLECULAR CHARACTERIZATION [J].

MOBLEY, HLT ;

HAUSINGER, RP .

MICROBIOLOGICAL REVIEWS, 1989, 53 (01) :85-108