ENZYME NANOBAND ELECTRODES

被引：15

作者：

WANG, J ^{[1
]}

NASER, N ^{[1
]}

RENSCHLER, CL ^{[1
]}

机构：

[1] SANDIA NATL LABS,DIV 1812,ALBUQUERQUE,NM 87185

来源：

ANALYTICAL LETTERS | 1993年 / 26卷 / 07期

关键词：

ENZYME MICROSENSORS; CARBON FILMS; GLUCOSE;

D O I：

10.1080/00032719308017416

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Enzyme nanoelectrodes have been constructed by immobilizing glucose oxidase, alcohol oxidase or tyrosinase onto ultrathin carbon films (of 35-50 nm thickness). The enzyme immobilization is accomplished via entrapment within electropolymerized poly(o-phenylenediamine) coatings. Cyclic voltammetry and controlled-potential amperometry are used to characterize the performance of the new nanoscopic biosensors under different preparation and operation conditions. The resulting electrodes offer convenient and rapid measurements of millimolar substrate concentrations, and (to the best of our knowledge) are the smallest enzyme probes reported to date.

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页码：1333 / 1346

页数：14

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