TRANSIENT-PHASE KINETICS OF ENZYME INACTIVATION INDUCED BY SUICIDE SUBSTRATES - ENZYMES INVOLVING 2 SUBSTRATES

被引：12

作者：

VARON, R

GARCIA, M

GARCIACANOVAS, F

TUDELA, J

机构：

[1] UNIV MURCIA, DEPT BIOQUIM & BIOL MOLEC, E-30001 MURCIA, SPAIN

[2] ESCUELA UNIV POLITECN ALBACETE, DEPT QUIM, LA MANCHA, SPAIN

来源：

JOURNAL OF MOLECULAR CATALYSIS | 1990年 / 59卷 / 01期

关键词：

D O I：

10.1016/0304-5102(90)85091-U

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

This paper reports the kinetic study of reaction mechanisms with enzyme inactivation induced by a suicide substrate for enzymes involving two substrates. A transient-phase approach has been developed that enables explicit equations with one or more significant exponentials to be obtained, thereby showing the dependence of product concentration on time. Methods for discrimination between different mechanisms for substrate binding sequences have been proposed, as well as an experimental design to determine the corresponding parameters and kinetic constants. © 1990.

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页码：97 / 118

页数：22

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