TRANSIENT-PHASE KINETICS OF ENZYME INACTIVATION INDUCED BY SUICIDE SUBSTRATES - ENZYMES INVOLVING 2 SUBSTRATES

This paper reports the kinetic study of reaction mechanisms with enzyme inactivation induced by a suicide substrate for enzymes involving two substrates. A transient-phase approach has been developed that enables explicit equations with one or more significant exponentials to be obtained, thereby showing the dependence of product concentration on time. Methods for discrimination between different mechanisms for substrate binding sequences have been proposed, as well as an experimental design to determine the corresponding parameters and kinetic constants. © 1990.