BONE-INDUCING ACTIVITY OF MATURE BMP-2B PRODUCED FROM A HYBRID BMP-2A/2B PRECURSOR

被引：158

作者：

HAMMONDS, RG ^{[1
]}

SCHWALL, R ^{[1
]}

DUDLEY, A ^{[1
]}

BERKEMEIER, L ^{[1
]}

LAI, C ^{[1
]}

LEE, J ^{[1
]}

CUNNINGHAM, N ^{[1
]}

REDDI, AH ^{[1
]}

WOOD, WI ^{[1
]}

MASON, AJ ^{[1
]}

机构：

[1] NIH, BONE CELL BIOL SECT, BETHESDA, MD 20892 USA

来源：

MOLECULAR ENDOCRINOLOGY | 1991年 / 5卷 / 01期

关键词：

D O I：

10.1210/mend-5-1-149

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The human osteoinductive proteins BMP-2a and BMP-2b have been cloned and expressed in mammalian cells. In order to improve expression levels we examined the role of the proregion in assembly and export. Use of the BMP-2a proregion combined with the mature region of BMP-2b leads to dramatically improved expression of mature BMP-2b. Mature BMP-2a has been purified to near homogeneity from the BMP-2a/2b hybrid, and its structural properties and biological activity determined. Recombinant mature BMP-2b homodimer elicits bone formation in vivo.

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页码：149 / 155

页数：7

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