STUDIES ON THE UPTAKE OF N-METHYLISOQUINOLINIUM ION INTO RAT STRIATAL SLICES USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORIMETRIC DETECTION

被引：9

作者：

HIRATA, Y ^{[1
]}

MINAMI, M ^{[1
]}

NAOI, M ^{[1
]}

NAGATSU, T ^{[1
]}

机构：

[1] NAGOYA UNIV,SCH MED,DEPT BIOCHEM,NAGOYA,AICHI 466,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 503卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(01)81500-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and sensitive procedure for the measurement of N-methylisoquinolinium ion (NMIQ+), a putative neurotoxin, was devised using high-performance liquid chromatography (HPLC) with fluorescence detection. Separation of NMIQ+ was carried out by gel filtration and reversed-phase HPLC on a column of hydrophilic polymer gels (Asahipak GS-302H). The method was sensitive enough to measure 50 fmol of NMIQ+. Uptake of NMIQ+ into rat striatal slices was confirmed by this method. © 1990.

引用

页码：189 / 195

页数：7

共 15 条

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