HIGH-EFFICIENCY TRANSFORMATION OF BACILLUS-SUBTILIS NB22, AN ANTIFUNGAL ANTIBIOTIC ITURIN PRODUCER, BY ELECTROPORATION

被引：35

作者：

MATSUNO, Y ^{[1
]}

ANO, T ^{[1
]}

SHODA, M ^{[1
]}

机构：

[1] TOKYO INST TECHNOL,RESOURCES UTILIZAT RES LAB,4259 NAGATSUTA,MIDORI KU,YOKOHAMA,KANAGAWA 227,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1992年 / 73卷 / 04期

关键词：

D O I：

10.1016/0922-338X(92)90179-X

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

High-voltage electroporation was used to transform Bacillus subtilis NB22, an antifungal antibiotic producer, reaching the efficiency of 10(7) transformants/mu-g plasmid DNA. Transformation frequency was dependent on the composition of the electroporation solution, the electrical field strength and the cell concentration. Addition of polyethylene glycol (PEG) and mannitol in the transformation solution was critical for a high efficiency of transformation.

引用

页码：261 / 264

页数：4

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