INDUCTION OF HAPLOID PLANTS IN ONION (ALLIUM-CEPA L) BY INVITRO CULTURE OF UNPOLLINATED OVULES

被引：49

作者：

CAMPION, B

ALLONI, C

机构：

[1] Research Institute for Vegetable Crops, Montanaso Lombardo, Milano, 20075

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1990年 / 20卷 / 01期

关键词：

Allium cepa L; embryo development; gynogenesis; haploids; onion; ovule culture;

D O I：

10.1007/BF00034750

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Haploid plants have been obtained from unpollinated ovules of onion cultured in vitro as here reported for the first time. Callus was not formed at any step of the process and the young developing embryos sprouted by splitting the ovules. From a total of about 26000 cultured ovules, 13 embryos were obtained and 7 of them developed into plants. Cytological observation of root apexes showed that 3 embryos out of 7 regenerated haploid plants (8 chromosomes). The yield of embryo induction was quite low (0.28% the best treatment) so further experiments are in progress to increase it. © 1990 Kluwer Academic Publishers.

引用

页码：1 / 6

页数：6

共 13 条

[1]

Campion B., 1984, 3rd Eucarpia Alliumsymposium, Wageningen, 4-6 September 1984., P110

[2]

DEBEAUVILLE MA, 1980, CR ACAD SCI D NAT, V290, P489

[3] PRODUCTION OF HAPLOID PLANTS OF SUNFLOWER (HELIANTHUS-ANNUUS L) BY INVITRO CULTURE OF NON-FERTILIZED OVARIES AND OVULES [J].