GASTRIN AND EPIDERMAL GROWTH-FACTOR INDUCTION OF ORNITHINE DECARBOXYLASE IN RAT COLONIC EXPLANTS

被引：18

作者：

ARLOW, FL

WALCZAK, SM

MOSHIER, JA

PIETRUK, T

MAJUMDAR, APN

机构：

[1] VET ADM MED CTR,RES SERV,151,ALLEN PK,MI 48101

[2] WAYNE STATE UNIV,SCH MED,DEPT MED,DIV GASTROENTEROL,DETROIT,MI 48201

[3] WAYNE STATE UNIV,DEPT NUTR & FOOD SCI,DETROIT,MI 48202

来源：

LIFE SCIENCES | 1990年 / 46卷 / 11期

关键词：

D O I：

10.1016/0024-3205(90)90065-Y

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

An organ culture system was utilized to examine the effect of gastrin (G-17-I) and epidermal growth factor (EGF) on colonic mucosal ornithine decarboxylase (ODC) activity, and the expression of the ODC gene. Exposure of colonic mucosal explants to either gastrin or EGF (50-500 ng/ml) for only 4 h resulted in a profound stimulation (150-600%) in ODC activity over the basal level. These increases were essentially abolished by difluoromethylornithine (DFMO; 2 nmol/ml) or CaCl2 (2 umol/ml). Gastrin also activated the ODC gene in the colonic mucosa as evidenced by increased steady-state ODC mRNA levels in the colonic mucosal explants after 4 h exposure to the hormone, when compared with the controls. It is concluded that colonic mucosal ODC is responsive to both gastrin and EGF. © 1990.

引用

页码：777 / 784

页数：8