CELL-MEDIATED LYMPHOLYSIS - EXAMINATION OF HLA GENETIC FINE-STRUCTURE AND COMPLEMENTATION USING CYTOTOXIC LYMPHOCYTES

Human cytotoxic lymphocytes (CTL), sensitized in vitro in one‐way mixed lymphocyte cultures between two unrelated individuals, have been used to study target cell determinants. One pair of CTL shows strong cell‐mediated lympholysis responses against the specific target cells as well as against third‐party target cells obtained from unrelated individuals. Testing of sixty target cells shows a significant association between the two CTL with a correlation coefficient of 0.67 in the panel. The target determinant recognized by the CTL is not an HLA‐A,B,C or D specificity, as defined by standard serological and cellular reagents. Segregation studies in a large family reveal that the new specificity defined by the CTL is controlled by HLA, and its recognition may be restricted by the HLA complex. Copyright © 1978 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim