HOMOLOGY OF PLASMIDS IN STRAINS OF UNICELLULAR CYANOBACTERIA

被引：93

作者：

VANDENHONDEL, CAMJJ ^{[1
]}

KEEGSTRA, W ^{[1
]}

BORRIAS, WE ^{[1
]}

VANARKEL, GA ^{[1
]}

机构：

[1] STATE UNIV UTRECHT,INST MOLEC BIOL,UTRECHT,NETHERLANDS

来源：

PLASMID | 1979年 / 2卷 / 03期

关键词：

D O I：

10.1016/0147-619X(79)90016-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Six strains of unicellular cyanobacteria were examined for the presence of plasmids. Analysis of lysates of these strains by CsCl-ethidium bromide density centrifugation yielded a major chromosomal DNA band and a minor band containing covalently closed circular plasmid DNA, as shown by electron microscopy and agarose gel electrophoresis. The sizes of the various plasmid species were determined; in each of the Synechococcus strains 6301, 6707, and 6908 two plasmid species were found with molecular weights of 5.3 × 106 and 32.7 × 106. Synechococcus strain 7425 had two plasmids of molecular weight 5.4 × 106 and 24 × 106. Synechococcus strain 6312 and Synechocystis strain 7005 each contained one plasmid species with molecular weight of 15.9 × 106 and 2.0 × 106, respectively. Restriction enzyme analysis revealed identical cleavage patterns for the plasmids of identical molecular weight. © 1979.

引用

页码：323 / 333

页数：11

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