EQUATORIAL SPLIT OF HOLO-CHAPERONIN FROM THERMUS-THERMOPHILUS BY ATP AND K+

被引：28

作者：

ISHII, N

TAGUCHI, H

SASABE, H

YOSHIDA, M

机构：

[1] TOKYO INST TECHNOL,RESOURCES UTILIZAT LAB R-1,YOKOHAMA,KANAGAWA 226,JAPAN

[2] INST PHYS & CHEM RES,FRONTIER RES PROGRAM,WAKO,SAITAMA 35101,JAPAN

来源：

FEBS LETTERS | 1995年 / 362卷 / 02期

基金：

日本科学技术振兴机构;

关键词：

CHAPERONIN; SPLIT OF; GROEL; THERMUS THERMOPHILUS;

D O I：

10.1016/0014-5793(95)00222-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Holo-chaperonin molecule from Thermus thermophilus is a bullet-shaped particle whose cylinder part and round top are composed of two stacked rings of the cpn60 heptamer and a single ring of the cpn10 heptamer, respectively, We found that it splits at the plane between two cpn60 rings into two halves under physiological conditions, that is, in the presence of ATP (but not AMP-PNP, ADP) + K+ (but not Na+) at 60 degrees C, This equatorial split could be functionally important although it has not been considered in any current mechanistic model of chaperonin functioning.

引用

页码：121 / 125

页数：5

共 24 条

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