SOLID-PHASE DERIVATIZATION OF AMINO-ACIDS AND PEPTIDES IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

This paper presents solid-phase derivatization of amino acids and peptides on a hydrophobic polymeric reagent. Using cationic surfactants as a basic pH, the negatively charged amino acids and peptides are ion-paired and derivatized by a 9-fluoreneacetyl tagged polymeric reagent. Using an off-line derivatization approach, the effects of the ion-pair reagent, buffer pH, reaction temperature, etc., on the derivatization are evaluated. Derivatization of peptides of enzymatically digested cytochrome c is included to show the feasibility of solid-phase derivatization to peptide mapping.