CLONING AND EXPRESSION OF A LIMONENE DEGRADATION PATHWAY FROM BACILLUS-STEAROTHERMOPHILUS IN ESCHERICHIA-COLI

被引：28

作者：

CHANG, HC ^{[1
]}

GAGE, DA ^{[1
]}

ORIEL, PJ ^{[1
]}

机构：

[1] MICHIGAN STATE UNIV,DEPT BIOCHEM,E LANSING,MI 48824

来源：

JOURNAL OF FOOD SCIENCE | 1995年 / 60卷 / 03期

关键词：

CLONING; LIMONENE DEGRADATION; ESCHERICHIA-COLI; BACILLUS STEAROTHERMOPHILUS;

D O I：

10.1111/j.1365-2621.1995.tb09824.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The pathway encoding limonene catabolism was cloned as a 9.6kb chromosomal fragment from Bacillus stearothermophilus strain BR388 into Escherichia coli. It conferred growth on limonene as a sole carbon source and bioproduction of alpha-terpineol, perillyl alcohol, and perillyl aldehyde. Incubation of the recombinant with perillyl alcohol resulted in formation of perillyl aldehyde and perillic acid. Catabolic pathways apparently affected the microbial toxicity of limonene.

引用

页码：551 / 553

页数：3