SENSITIVE MICROMETHOD FOR COLUMN LIQUID-CHROMATOGRAPHIC DETERMINATION OF FLUOXETINE AND NORFLUOXETINE IN HUMAN PLASMA

被引：36

作者：

THOMARE, P ^{[1
]}

WANG, K ^{[1
]}

VANDERMEERSCHMOUGEOT, V ^{[1
]}

DIQUET, B ^{[1
]}

机构：

[1] HOP PITIE,DEPT PHARMACOL CLIN,PHARMACOCINET LAB,47 BLVD HOP,F-75651 PARIS 13,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 583卷 / 02期

关键词：

D O I：

10.1016/0378-4347(92)80555-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid, selective and sensitive micromethod has been developed for the determination of fluoxetine (FLU) and its demethylated metabolite norfluoxetine (N-FLU) using a 250-mul plasma sample and column liquid chromatography with ultraviolet detection at 226 nm. The limit of detection is 2.0 ng/ml for both FLU and N-FLU. Peak-height ratios are linear over a concentration range of 10-800 and 10-1000 ng/ml for FLU and N-FLU, respectively. Acceptable coefficients of variation are demonstrated for both within-run and day-to-day assays. Selected drugs were checked for interference. The method, which requires a very small volume of plasma, is sensitive enough for pharmacokinetic studies in animals, clinical pharmacology studies and drug monitoring in children or adult patients.

引用

页码：217 / 221

页数：5

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