NEW PH-SENSITIVE AMINOXYLS - APPLICATION TO THE STUDY OF BIOMEMBRANE TRANSPORT PROCESSES

被引：11

作者：

BALAKIREV, M ^{[1
]}

KHRAMTSOV, V ^{[1
]}

机构：

[1] NOVOSIBIRSK CHEM KINET & COMBUST INST, NOVOSIBIRSK 630090, RUSSIA

来源：

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2 | 1993年 / 11期

关键词：

D O I：

10.1039/p29930002157

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

An EPR method for studying transmembrane proton transport using pH-sensitive aminoxyl radicals has been developed for applications in the pH region 4-7. To this end pH-sensitive spin-labelled dextran (pK = 6.1) and sulfonic acid (pK = 5.25), which do not penetrate phospholipid membranes, were synthesized. Using EPR spectroscopy these probes were used to measure the kinetics of pH variations inside large unilamellar phospholipid vesicles after creation. of a transmembrane proton gradient. Membrane permeability coefficients for H+ (5 x 10(-4) cm s-1), Cl-(10(-10) cm s-1) and Tl+ (10(-8) cm s-1) were estimated.

引用

页码：2157 / 2160

页数：4