THE DISULFIDE BRIDGE PATTERN OF SNAKE-VENOM DISINTEGRINS, FLAVORIDIN AND ECHISTATIN

被引：64

作者：

CALVETE, JJ

WANG, YQ

MANN, K

SCHAFER, W

NIEWIAROWSKI, S

STEWART, GJ

机构：

[1] MAX PLANCK INST BIOCHEM,W-8033 MARTINSRIED,GERMANY

[2] TEMPLE UNIV,HLTH SCI CTR,SCH MED,DEPT PHYSIOL,PHILADELPHIA,PA 19140

[3] TEMPLE UNIV,HLTH SCI CTR,SCH MED,SOL SHERRY THROMBOSIS RES CTR,PHILADELPHIA,PA 19140

来源：

FEBS LETTERS | 1992年 / 309卷 / 03期

关键词：

INHIBITOR OF INTEGRIN; DISINTEGRIN; DISULFIDE BRIDGE; ECHISTATIN; FLAVORIDIN;

D O I：

10.1016/0014-5793(92)80797-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Flavoridin and echistatin, isolated from the venom of Trimeresurus flavoviridis and Echis carinatus, respectively, belong to the disintegrin family of integrin beta-1 and beta-3 inhibitors of low molecular weight RGD-containing, cysteine-rich peptides. Since disulfide bonds are critical for expression of biological activity, we sought to determine their location in these two proteins. In flavoridin, direct evidence for the existence of linkage between Cys4-Cys19 and between Cys45 and Cys64 was obtained by analysis of proteolytic products, and indirect evidence suggests links between Cys6-Cys14 and Cys13-Cys36. In echistatin, links between Cys8-Cys37 and Cys20-Cys39 were identified by direct chemical analysis.

引用

页码：316 / 320

页数：5