ISOLATION AND PURIFICATION OF DIGALACTOSYLDIACYLGLYCEROLS

A convenient method for digalactosyldiacylglycerols from been developed using solid phase extraction. Commercial cartridges were utilized to prepare 100 mg fractions of galactolipids containing more than 97 % of digalactosyldiacylglycerol. The purity of the fractions was monitored by an isocratic normal phase analytical HPLC procedure, which was optimized using factorial design. The optimized system was successfully scaled up to a semi-preparative HPLC system with a capacity of about 10 mg/injection which was used to further purify the digalactosyldiacylglycerol fraction.