HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF WHEAT-FLOUR LIPIDS USING AN EVAPORATIVE LIGHT-SCATTERING DETECTOR

A high-performance liquid chromatographic method which utilized an evaporative light scattering detector for separation of starch and non-starch lipids of unbleached soft red winter wheat flour is described. Separation of the major starch and non-starch lipids was achieved in 60 min using a Lichrosorb Si-60 silica cartridge system and ternary gradient system. The evaporative light scattering detector gave a flat stable baseline, reproducible results and also eliminated the ''solvent fronts'' in which peaks of interest would have co-eluted. The technique was found useful in identifying the lipids and their relative mass percentages that were present in the flour.