IN-SITU HYBRIDIZATION APPROACH AT INFRAGENIC LEVEL ON METAPHASE CHROMOSOMES

被引：10

作者：

LEMIEUX, N

MALFOY, B

FETNI, R

MULERIS, M

VOGT, N

RICHER, CL

DUTRILLAUX, B

机构：

[1] INST CURIE,CNRS,URA 620,BIOL STRUCT & MUTAGENESE CHROMOSOM SECT,F-75231 PARIS 05,FRANCE

[2] UNIV MONTREAL,FAC MED,DEPT PATHOL,MONTREAL H3C 3J7,PQ,CANADA

[3] UNIV MONTREAL,FAC MED,DEPT ANAT,MONTREAL H3C 3J7,PQ,CANADA

来源：

CYTOGENETICS AND CELL GENETICS | 1994年 / 66卷 / 02期

关键词：

D O I：

10.1159/000133678

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Analysis of the retinoblastoma locus (RB1) by in situ hybridization at the infragenic level was done using human chromosomes. A high level of resolution was attained with nonisotopic in situ hybridization on banded chromosomes in both fluorescence and electron microscopy. DNA sequences less than 100 kb apart could be positioned on band 13q14 in the order expected from molecular mapping. These observations suggest that the DNA target of hybridized probes on metaphase chromosomes may correspond to nucleoprotein loops giving a resolution comparable to that obtained in interphase nuclei.

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页码：107 / 112

页数：6

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