STRUCTURE OF THE GLYCOSYLPHOSPHATIDYLINOSITOL MEMBRANE ANCHOR OF HUMAN PLACENTAL ALKALINE-PHOSPHATASE

被引：49

作者：

REDMAN, CA

THOMASOATES, JE

OGATA, S

IKEHARA, Y

FERGUSON, MAJ

机构：

[1] UNIV DUNDEE,DEPT BIOCHEM,DUNDEE DD1 4HN,SCOTLAND

[2] FUKUOKA UNIV,SCH MED,DEPT BIOCHEM,JONAN KU,FUKUOKA 81401,JAPAN

来源：

BIOCHEMICAL JOURNAL | 1994年 / 302卷

基金：

英国惠康基金;

关键词：

D O I：

10.1042/bj3020861

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase was isolated by exhaustive proteolysis followed by hydrophobic interaction chromatography. The resulting glycocylphosphatidylinositol-peptide was subjected to compositional analysis and chemical and enzymic modifications. The neutral-glycan fraction, prepared by dephosphorylation followed by HNO2 deamination and reduction, was sequenced using exoglycosidases and acetolysis. The phosphatidylinositol moiety was analysed by fast-atom bombardment mass spectrometry and gas chromatography-mass spectrometry. Taken together the data suggest the structure, Thr-Asp-ethanolamine-PO4-man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN-(sn-1-O-alkyl-2-O-acylglycerol-3-PO4-1-myo-D-inositol), which contains an additional ethanolamine phosphate group at an unknown position.

引用

页码：861 / 865

页数：5

共 29 条

[11] PHOSPHATIDYLINOSITOL ANCHOR OF HELA-CELL ALKALINE-PHOSPHATASE [J].