A COMPARISON OF 6 DIFFERENT PLATING MEDIA USED IN THE ISOLATION OF SALMONELLA

被引：24

作者：

WARBURTON, DW

BOWEN, B

KONKLE, A

CRAWFORD, C

DURZI, S

FOSTER, R

FOX, C

GOUR, L

KROHN, G

LACASSE, P

LAMONTAGNE, G

MCDONAGH, S

ARLING, V

MACKENZIE, J

TODD, ECD

OGGEL, J

PLANTE, R

SHAW, S

TIWARI, NP

TROTTIER, Y

WHEELER, BD

机构：

[1] HLTH & WELF CANADA,HPB,DIV FIELD OPERAT,BURNABY V5G 4P2,BC,CANADA

[2] FISHERIES & OCEANS CANADA,TORONTO M6N 3E3,ON,CANADA

[3] HLTH & WELF CANADA,HPB,FIELD OPERAT DIRECTORATE,WINNIPEG R2J 3Y1,MB,CANADA

[4] HLTH & WELF CANADA,HPB,FIELD OPERAT DIRECTORATE,DARTMOUTH B2Y 3Z7,NS,CANADA

[5] HLTH & WELF CANADA,HPB,FIELD OPERAT DIRECTORATE,LONGUEUIL J4K 1C7,PQ,CANADA

[6] SASKATCHEWAN AGR & FOOD,REGINA VET LAB,REGINA S4S 0B1,SK,CANADA

[7] MINIST AGR PECHERIES & ALIMENTAT QUEBEC,LABS EXPERTISES & ANAL ALIMENTAIRES ST FOY,ST FOY G1P 2W8,PQ,CANADA

[8] MINIST AGR PECHERIES & ALIMENTAT QUEBEC,LABS EXPERTISES & ANAL ALIMENTAIRES ST HYACINTHE,ST HYACINTHE J2S 2M2,PQ,CANADA

[9] AGR CANADA,LAB SERV DIV W,CALGARY T2L 2L1,AB,CANADA

[10] HLTH & WELF CANADA,BUR MICROBIAL HAZARDS,DIV RES,OTTAWA K1A 0L2,ONTARIO,CANADA

[11] AGR CANADA,DIV LAB SERV,OTTAWA K1A 0C6,ONTARIO,CANADA

[12] COMMUNAUTE URBAIN MONTREAL,SERV ENVIRONNEMENT,MONTREAL H4N 2T2,PQ,CANADA

[13] FISHERIES & OCEANS CANADA,HALIFAX B3J 2S7,NS,CANADA

[14] ALBERTA DEPT AGR,SERV BRANCH,FOOD LAB,EDMONTON T6H 4P2,AB,CANADA

[15] AGR CANADA,HYG VET LAB,ST HYACINTHE J2S 8E3,PQ,CANADA

[16] FISHERIES & OCEANS CANADA,FISH INSPECT LAB,ST JOHNS A1C 5X1,NF,CANADA

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 1994年 / 22卷 / 04期

关键词：

COMPARATIVE COLLABORATIVE STUDY; PLATING MEDIA; ISOLATION PROCEDURE; SALMONELLA;

D O I：

10.1016/0168-1605(94)90179-1

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Seventeen Canadian Federal, Provincial and Public Health Laboratories took part in different phases of a comparative/collaborative study that evaluated rapid methods to the standard Health Protection Branch (HPB) method for the detection of Salmonella. A variety of commercial media were tested, including Brilliant Green Sulpha Agar, Bismuth Sulphite Agar, Hektoen Enteric Agar, Xylose Lysine Deoxycholate Agar, EF-18 Agar and Rambach Agar. Each laboratory compared up to six of these different plating media. Plating of 123 salmonellae cultures and 28 artificially-inoculated foods showed the recovery of Salmonella spp. on the six plating media to be within one log. Therefore, quantitative testing of the media showed them to be comparable in the recovery of salmonellae. Qualitative testing of the six media during the comparative/collaborative study of various methods showed that EF-18 Agar recovered the greatest number of isolates. Hektoen Enteric Agar ranked second, with the other agars being comparable in their recovery of Salmonella spp. Problems with the various media are summarized. Based on our results and those of other researchers, it is recommended that Bismuth Sulphite Agar be compulsory and that at least one other agar be used for newly developed cultural procedures.

引用

页码：277 / 289

页数：13

共 35 条

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