MOLECULAR MECHANISMS OF OLFACTORY RECEPTION .4. SOME BIOCHEMICAL CHARACTERISTICS OF THE CAMPHOR RECEPTOR FROM RAT OLFACTORY EPITHELIUM

被引:49
作者
FESENKO, EE
NOVOSELOV, VI
KRAPIVINSKAYA, LD
机构
[1] Institute of Biological Physics, Academy of Sciences, the USSR, Pushchino
关键词
(Rat olfactory mucosa); Camphor; Olfaction; Receptor;
D O I
10.1016/0304-4165(79)90446-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Some parameters of the receptor element from the rat olfactory epithelium are evaluated; it is characterized by a high affinity for camphor (KD = 1.5 · 10-9 M). Triton X-100 has no marked effect on the binding of [3H]camphor. Neither RNAase nor phospholipase C affected [3H]camphor-binding activity. Pronase and trypsin abolished [3H]camphor binding activity by 65 and 40%, respectively. Sulfhydryl reagents decrease the binding of [3H]camphor by a factor of 5-8. The isoelectric point of the receptor solubilized with Triton X-100 is 4.8, as determined by isoelectric focusing. The molecular weight of the receptor as determined by gel electrophoresis is about 120 000. It is proposed that the camphor receptor is a membrane protein containing sulfhydryl groups and playing a key role in olfactory reception. © 1979.
引用
收藏
页码:424 / 432
页数:9
相关论文
共 19 条
[2]   A THEORY OF QUALITY OF ODOURS [J].
DAVIES, JT .
JOURNAL OF THEORETICAL BIOLOGY, 1965, 8 (01) :1-&
[3]  
FESENKO EE, 1978, PREPRINT I BIOL PHYS, P1
[4]  
GETCHELL M L, 1972, Proceedings of the National Academy of Sciences of the United States of America, V69, P1494, DOI 10.1073/pnas.69.6.1494
[5]   OLFACTORY RESPONSES TO AMINO-ACIDS IN RAINBOW-TROUT, SALMO-GAIRDNERI [J].
HARA, TJ .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY, 1973, 44 (2A) :407-416
[6]   DEVELOPMENT OF MOUSE OLFACTORY VESICLES AND THEIR CELL CONTACTS - FREEZE-ETCHING STUDY [J].
KERJASCHKI, D ;
HORANDNER, H .
JOURNAL OF ULTRASTRUCTURE RESEARCH, 1976, 54 (03) :420-444
[7]  
KOYAMA M, 1972, NATURE, V236, P401
[8]   EXTRACTION, PARTIAL-PURIFICATION AND CHARACTERIZATION OF INSOLUBLE ESTROGEN-RECEPTOR FROM CHICK LIVER NUCLEI [J].
LEBEAU, MC ;
MASSOL, N ;
BAULIEU, EE .
FEBS LETTERS, 1974, 43 (01) :107-111
[9]  
LOWRY OH, 1951, J BIOL CHEM, V193, P265
[10]  
Maurer K, 2005, PRAXIS EVOZIERTEN PO