DNA-RFLP METHODS AND INTERPRETATION SCHEME FOR HLA-DR-TYPING AND DQ-TYPING

被引:68
作者
BIDWELL, JL [1 ]
BIGNON, JD [1 ]
机构
[1] LAB IMMUNOL LEUCOPLAQUETTAIRE & HISTOCOMPATIBIL FRANCE TRANSPLANT,NANTES,FRANCE
来源
EUROPEAN JOURNAL OF IMMUNOGENETICS | 1991年 / 18卷 / 1-2期
关键词
D O I
10.1111/j.1744-313X.1991.tb00003.x
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
DNA-restriction fragment length polymorphism (RFLP) typing is a well established and standard technique for identification of HLA-DR and DQ allotypes. We present a detailed review of methods for RFLP analysis and a scheme for interpretation of results. This scheme permits the routine identification of HLA-DR and DQ allotypes using the restriction endonucleases TaqI and either HindIII or MspI.
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页码:5 / 22
页数:18
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