MOLECULAR ANALYSIS OF 2-DIMENSIONAL PROTEIN CRYSTALLIZATION

被引：37

作者：

KU, AC

DARST, SA

ROBERTSON, CR

GAST, AP

KORNBERG, RD

机构：

[1] STANFORD UNIV,DEPT CELL BIOL,STANFORD,CA 94305

[2] STANFORD UNIV,DEPT CHEM ENGN,STANFORD,CA 94305

来源：

JOURNAL OF PHYSICAL CHEMISTRY | 1993年 / 97卷 / 12期

关键词：

D O I：

10.1021/j100114a030

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Two-dimensional crystals of streptavidin formed on lipid layers at the air-water interface were cross-linked to preserve their structure during transfer to a solid support. The morphology of the crystals viewed at the air-water interface by light microscopy could then be related to the molecular structure revealed in the electron microscope. The preferred growth direction of the crystals proved to be along one of two intersecting rows of intermolecular contacts. A secondary direction arises during a transition to dendritic growth.

引用

页码：3013 / 3016

页数：4

共 12 条

[1] 3-DIMENSIONAL STRUCTURE DETERMINATION BY ELECTRON-MICROSCOPY OF TWO-DIMENSIONAL CRYSTALS
AMOS, LA
HENDERSON, R
UNWIN, PNT
[J]. PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1983, 39 (03) : 183 - 231
[2] BENVEGNUE DJ, 1992, J PHYS CHEM-US, V967, P6820
[3] TWO-DIMENSIONAL CRYSTALS OF ESCHERICHIA-COLI RNA-POLYMERASE HOLOENZYME ON POSITIVELY CHARGED LIPID LAYERS
DARST, SA
RIBI, HO
PIERCE, DW
KORNBERG, RD
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1988, 203 (01) : 269 - 273
[4] 2-DIMENSIONAL CRYSTALS OF STREPTAVIDIN ON BIOTINYLATED LIPID LAYERS AND THEIR INTERACTIONS WITH BIOTINYLATED MACROMOLECULES
DARST, SA
AHLERS, M
MELLER, PH
KUBALEK, EW
BLANKENBURG, R
RIBI, HO
RINGSDORF, H
KORNBERG, RD
[J]. BIOPHYSICAL JOURNAL, 1991, 59 (02) : 387 - 396
[5] Green N M, 1975, Adv Protein Chem, V29, P85, DOI 10.1016/S0065-3233(08)60411-8
[6] FUNDAMENTALS OF DENDRITIC SOLIDIFICATION .2. DEVELOPMENT OF SIDEBRANCH STRUCTURE
HUANG, SC
GLICKSMAN, ME
[J]. ACTA METALLURGICA, 1981, 29 (05): : 717 - 734
[7] LATERAL DIFFUSION OF PHOSPHOLIPIDS IN A VESICLE MEMBRANE
KORNBERG, RD
MCCONNEL.HM
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1971, 68 (10) : 2564 - &
[8] Two-dimensional crystals of proteins on lipid layers
Kornberg, Roger D.
Darst, Seth A.
[J]. CURRENT OPINION IN STRUCTURAL BIOLOGY, 1991, 1 (04) : 642 - 646
[9] DENDRITIC GROWTH OF 2-DIMENSIONAL PROTEIN CRYSTALS
KU, AC
DARST, SA
KORNBERG, RD
ROBERTSON, CR
GAST, AP
[J]. LANGMUIR, 1992, 8 (10) : 2357 - 2360
[10] IMPROVED TRANSFER OF 2-DIMENSIONAL CRYSTALS FROM THE AIR-WATER-INTERFACE TO SPECIMEN SUPPORT GRIDS FOR HIGH-RESOLUTION ANALYSIS BY ELECTRON-MICROSCOPY
KUBALEK, EW
KORNBERG, RD
DARST, SA
[J]. ULTRAMICROSCOPY, 1991, 35 (3-4) : 295 - 304

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