LITHIUM-CHLORIDE EFFECT ON PHENYLETHYL-B-D-GALACTOSIDE SYNTHESIS BY ASPERGILLUS-ORYZAE B-D-GALACTOSIDASE IN THE PRESENCE OF HIGH LACTOSE CONCENTRATION

被引：15

作者：

FORTUN, Y ^{[1
]}

COLAS, B ^{[1
]}

机构：

[1] FAC SCI & TECH NANTES,CTR RECH BIOL & PHYSICOCHIM CELLULAIRES,BIOCHIM LAB 2,F-44072 NANTES 03,FRANCE

来源：

BIOTECHNOLOGY LETTERS | 1991年 / 13卷 / 12期

关键词：

D O I：

10.1007/BF01022087

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Phenylethyl-beta-D-galactoside (PEGal) synthesis by transglycosylation can be catalysed by Aspergillus oryzae beta-galactosidase and was studied using high concentrations of glycosyl donor (lactose). The amount of PEGal formed reached a maximum value after 10h in the presence of 0.5M lactose. Beyond this time, PEGal was slowly hydrolysed. LiCl between 0.4M and 0.7M in the reaction medium prevented the progressive disappearance of the product.

引用

页码：863 / 866

页数：4

共 20 条

[1] ENZYMIC-SYNTHESIS OF DISACCHARIDES BY USE OF THE REVERSED HYDROLYSIS ACTIVITY OF BETA-D-GALACTOSIDASES
AJISAKA, K
FUJIMOTO, H
NISHIDA, H
[J]. CARBOHYDRATE RESEARCH, 1988, 180 (01) : 35 - 42
[2] THE SYNTHESIS OF OLIGOSACCHARIDES BY THE REVERSED HYDROLYSIS REACTION OF BETA-GLUCOSIDASE AT HIGH SUBSTRATE CONCENTRATION AND AT HIGH-TEMPERATURE
AJISAKA, K
NISHIDA, H
FUJIMOTO, H
[J]. BIOTECHNOLOGY LETTERS, 1987, 9 (04) : 243 - 248
[3] REGIOSELECTIVE SYNTHESIS OF TRISACCHARIDES BY USE OF A REVERSED HYDROLYSIS ACTIVITY OF ALPHA-D-GALACTOSIDASE AND BETA-D-GALACTOSIDASE
AJISAKA, K
FUJIMOTO, H
[J]. CARBOHYDRATE RESEARCH, 1989, 185 (01) : 139 - 146
[4] CAER D, 1990, SCI ALIMENT, V10, P465
[5] STEREOSPECIFIC CHEMOENZYMATIC SYNTHESIS OF GALACTOPYRANOSYL-L-SERINE
CANTACUZENE, D
ATTAL, S
BAY, S
[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1991, 1 (04) : 197 - 200
[6] UNSATURATED SOLUTIONS OF SODIUM-CHLORIDE AS REFERENCE SOURCES OF WATER ACTIVITY AT VARIOUS TEMPERATURES
CHIRIFE, J
RESNIK, SL
[J]. JOURNAL OF FOOD SCIENCE, 1984, 49 (06) : 1486 - 1488
[7] COVALENT BINDING OF GLYCOSYL RESIDUES TO BOVINE CASEIN - EFFECTS ON SOLUBILITY AND VISCOSITY
COURTHAUDON, JL
COLAS, B
LORIENT, D
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1989, 37 (01) : 32 - 36
[8] SYNTHESIS OF GAL-BETA-1-3GLCNAC AND GAL-BETA-1-3GLCNAC-BETA-SET BY AN ENZYMATIC METHOD COMPRISING THE SEQUENTIAL USE OF BETA-GALACTOSIDASES FROM BOVINE TESTES AND ESCHERICHIA-COLI
HEDBYS, L
JOHANSSON, E
MOSBACH, K
LARSSON, PO
GUNNARSSON, A
SVENSSON, S
LONN, H
[J]. GLYCOCONJUGATE JOURNAL, 1989, 6 (02) : 161 - 168
[9] STUDIES OF THE REVERSED ALPHA-MANNOSIDASE REACTION IN HIGH-CONCENTRATIONS OF MANNOSE
JOHANSSON, E
HEDBYS, L
MOSBACH, K
LARSSON, PO
GUNNARSSON, A
SVENSSON, S
[J]. ENZYME AND MICROBIAL TECHNOLOGY, 1989, 11 (06) : 347 - 352
[10] COVALENT BINDING OF GLYCOSYL RESIDUES TO BETA-LACTOGLOBULIN - EFFECTS ON SOLUBILITY AND HEAT-STABILITY
KITABATAKE, N
CUQ, JL
CHEFTEL, JC
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1985, 33 (01) : 125 - 130

← 1 2 →