INTERACTION OF LYSINE-RICH HISTONES AND DNA

被引:131
作者
OLINS, DE
机构
[1] University of Tennessee, Oak Ridge Graduate School, Biomedical Sciences Biology Division, Oak Ridge
关键词
D O I
10.1016/0022-2836(69)90351-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Complexes of lysine-rich histones (f1) with native linear calf thymus DNA were prepared in soluble form by a salt-gradient dialysis method and examined by a variety of physico-chemical methods. Absorption spectroscopy and circular dichroism revealed minimal differences between DNA and histone-DNA complexes, suggesting that the DNA remains in the B conformation with histone present. Thermal denaturation and thermal renaturation studies indicated that the presence of histone resulted in a marked stabilization of DNA structure and permitted rapid renaturation of denatured regions. Maximum renaturation was effected with as few as 20 to 40 histone molecules per DNA molecule (107 mol. wt). Sedimentation velocity measurements of the complexes indicated that, at infinite dilution, the particles behave as single DNA molecules with associated histones. Evidence for concentration-dependent aggregation of the histone-DNA particles was also obtained. Dye-binding studies suggested that lysine-rich histone does not bind in the small groove of DNA; the presence of histone on DNA does not inhibit actinomycin binding. Furthermore, evidence was obtained indicating that histone might bind within the large groove of DNA; histone complexes with unglucosylated T2* DNA were less effective substrates for glucosylation by α-glucosyl transferase than was T2* DNA. © 1969.
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页码:439 / +
页数:1
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