FLUOROMETRIC INVIVO DETERMINATION OF BACILLUS-SUBTILIS AND PHAGE 2C DNA

被引:10
作者
DAXHELET, GA
KOHNEN, MM
COENE, MM
HOET, PP
机构
[1] Microbiology and Genetics Unit, University of Louvain Medical School, Institute of Cell Pathology
关键词
D O I
10.1016/0003-2697(90)90143-W
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The validity of in vivo fluorometric assays was ascertained for phage and bacterial DNA measurements. The following parameters were determined by this simple technique. The DNA content of dividing cells of Bacillus subtilis 168 2 was 2.65 times higher than in resting cells. Assuming that resting cells harbor 1 genomic equivalent, its Mr was estimated to be 4.4 × 109 Da. A polymerization rate during growth of 788,000 bp min-1/cell is accounted for by a multifork replication mechanism. Both phage and host DNA could be measured accurately during the lytic cycle. Phage 2C DNA synthesis proceeded at a linear rate of 5.2 genome equivalents min-1. © 1990.
引用
收藏
页码:116 / 119
页数:4
相关论文
共 18 条
[11]  
Kornberg A., 1980, DNA REPLICATION
[12]   ONE-STEP FLUOROMETRIC MICROASSAY OF DNA IN PROCARYOTES [J].
LEGROS, M ;
KEPES, A .
ANALYTICAL BIOCHEMISTRY, 1985, 147 (02) :497-502
[13]   REPLICATION OF VIRAL DNA IN SPO1-INFECTED BACILLUS-SUBTILIS .1. REPLICATIVE INTERMEDIATES [J].
LEVNER, MH ;
COZZARELLI, NR .
VIROLOGY, 1972, 48 (02) :402-+
[14]   THERMAL RENATURATION OF DEOXYRIBONUCLEIC ACIDS [J].
MARMUR, J ;
DOTY, P .
JOURNAL OF MOLECULAR BIOLOGY, 1961, 3 (05) :585-&
[15]   MOLECULAR WEIGHT OF DNA IN CHROMOSOMES OF E COLI AND B SUBTILIS [J].
MASSIE, HR ;
ZIMM, BH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1965, 54 (06) :1636-&
[16]   REPLICATION AND REPAIR OF DNA IN CELLS OF ESCHERICHIA-COLI TREATED WITH TOLUENE [J].
MOSES, RE ;
RICHARDSON, CC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1970, 67 (02) :674-+
[17]   SYMMETRIC REPLICATION OF BACILLUS-SUBTILIS CHROMOSOME [J].
QUINN, WG ;
SUEOKA, N .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1970, 67 (02) :717-&