CONCENTRATION-STEP AMPEROMETRIC SENSOR OF L-ASCORBIC-ACID USING CUCUMBER JUICE

被引：28

作者：

UCHIYAMA, S

UMETSU, Y

机构：

[1] Department of Environmental Engineering, Saitama Institute of Technology, Okabe, Saitama, 369-02

来源：

ANALYTICA CHIMICA ACTA | 1991年 / 255卷 / 01期

关键词：

AMPEROMETRY; ASCORBIC ACID; BIOSENSORS; ENZYME ELECTRODES; FRUIT JUICES; PLANT-TISSUE ELECTRODES;

D O I：

10.1016/0003-2670(91)85086-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

L-Ascorbic acid was determined by concentration-step amperometry using a thin layer of carbon felt impregnated with cucumber juice as an enzyme solution of ascorbate oxidase. The dilute fruit juice was added on top of the carbon felt and the decreased current peak caused by the enzymatic reaction was measured. The cucumber juice was prepared by filtration and centrifugal separation. The peak current was proportional to the concentration of L-ascorbic acid in the concentration range 2.5 x 10(-4) - 1.6 x 10(-3) M. The results obtained were in fairly good agreement with those obtained by liquid chromatography.

引用

页码：53 / 57

页数：5

共 15 条

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