PROTEIN SEPARATION WITH AQUEOUS AQUEOUS POLYMER SYSTEMS BY 2 TYPES OF COUNTERCURRENT CHROMATOGRAPHS

被引：43

作者：

SHIBUSAWA, Y ^{[1
]}

ITO, Y ^{[1
]}

机构：

[1] NHLBI,BIOPHYS CHEM LAB,BLDG 10,ROOM 7N322,BETHESDA,MD 20892

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 550卷 / 1-2期

关键词：

COIL PLANET CENTRIFUGE; MULTILAYER COILS; PHASE SYSTEMS; PERFORMANCE; COLUMNS;

D O I：

10.1016/S0021-9673(01)88575-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Two different types of counter-current chromatographs, the cross-axis coil planet centrifuge (X-axis CPC) and horizontal flow-through coil planet centrifuge (horizontal CPC), were evaluated for protein separation with an aqueous-aqueous two-phase polymer system. The sample solution, containing 10-200 mg each of cytochrome c, myoglobin, ovalbumin and hemoglobin in 2 ml of each phase was eluted with the lower phase. In both instruments, the effects of the flow-rate, revolution speed, and parameter beta-(helical diameters of the multilayer coil) on the protein separation were investigated. The best results were obtained from the X-axis CPC operated at 750 rpm and a flow-rate of 2.0 ml/min using a multilayer coil with a small helical diameter (beta = 0.25-0.60). Four protein samples were well resolved in less than 5 h.

引用

页码：695 / 704

页数：10

共 12 条

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SANDLIN, JL ;

ITO, Y .

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SUTHERLAND, IA ;

HEYWOODWADDINGTON, D ;

ITO, Y .

JOURNAL OF CHROMATOGRAPHY, 1987, 384 :197-207

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