BIOSYNTHESIS OF HUMAN PAPILLOMAVIRUS FROM A CONTINUOUS CELL-LINE UPON EPITHELIAL DIFFERENTIATION

被引:379
作者
MEYERS, C
FRATTINI, MG
HUDSON, JB
LAIMINS, LA
机构
[1] UNIV CHICAGO,HOWARD HUGHES MED INST,DEPT MOLEC GENET & CELL BIOL,CHICAGO,IL 60637
[2] UNIV CHICAGO,DEPT BIOCHEM & MOLEC BIOL,CHICAGO,IL 60637
关键词
D O I
10.1126/science.1323879
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The study of the human pathogen papillomaviruses (HPVs) has been hampered by the inability to propagate the virus in tissue culture. The addition of 12-O-tetradecanoyl phorbol-13-acetate to the media of organotypic (raft) cultures increased expression of physiological markers of keratinocyte differentiation and concomitantly induced production of virions. Capsid production was detected in differentiated suprabasal cells. Virions approximately 54 nanometers in size were observed by electron microscopy in raft tissue cross sections in the suprabasal layers. Virions purified through isopycnic gradients were found to contain type 31b DNA and exhibited an icosahedral shape similar to that of papillomaviruses found in clinical samples.
引用
收藏
页码:971 / 973
页数:3
相关论文
共 35 条
  • [21] MEYERS CLF, UNPUB
  • [22] THE CATALOG OF HUMAN CYTOKERATINS - PATTERNS OF EXPRESSION IN NORMAL EPITHELIA, TUMORS AND CULTURED-CELLS
    MOLL, R
    FRANKE, WW
    SCHILLER, DL
    GEIGER, B
    KREPLER, R
    [J]. CELL, 1982, 31 (01) : 11 - 24
  • [23] MORIN C, 1980, ACTA CYTOL, V24, P82
  • [24] THE 50-KDALTON AND 58-KDALTON KERATIN CLASSES AS MOLECULAR MARKERS FOR STRATIFIED SQUAMOUS EPITHELIA - CELL-CULTURE STUDIES
    NELSON, WG
    SUN, TT
    [J]. JOURNAL OF CELL BIOLOGY, 1983, 97 (01) : 244 - 251
  • [25] PFISTER H, 1987, PAPILLOMAVIRUSES HUM, P1
  • [26] CONDYLOMATA OF THE UTERINE CERVIX AND KOILOCYTOSIS OF CERVICAL INTRA-EPITHELIAL NEOPLASIA
    PILOTTI, S
    RILKE, F
    DEPALO, G
    DELLATORRE, G
    ALASIO, L
    [J]. JOURNAL OF CLINICAL PATHOLOGY, 1981, 34 (05) : 532 - 541
  • [27] CHARACTERIZATION OF PICHINDE VIRUS-INFECTION OF CELLS OF THE MONOCYTIC LINEAGE
    POLYAK, SJ
    RAWLS, WE
    HARNISH, DG
    [J]. JOURNAL OF VIROLOGY, 1991, 65 (07) : 3575 - 3582
  • [28] RADER JS, 1990, ONCOGENE, V5, P571
  • [29] SALZMAN NP, 1987, PAPOVAVIRIDAE, V2
  • [30] PRODUCTION OF HUMAN PAPILLOMAVIRUS TYPE-16 VIRIONS IN A KERATINOCYTE CELL-LINE
    STERLING, J
    STANLEY, M
    GATWARD, G
    MINSON, T
    [J]. JOURNAL OF VIROLOGY, 1990, 64 (12) : 6305 - 6307