CHARACTERIZATION OF AN ENDOGENOUS METALLOPROTEINASE IN HUMAN VITREOUS

被引：76

作者：

BROWN, D ^{[1
]}

HAMDI, H ^{[1
]}

BAHRI, S ^{[1
]}

KENNEY, MC ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,CEDARS SINAI MED CTR,SCH MED,CEDARS SINAI RES INST,DEPT SURG,LOS ANGELES,CA 90048

来源：

CURRENT EYE RESEARCH | 1994年 / 13卷 / 09期

关键词：

MATRIX METALLOPROTEINASE; MMP-2; VITREOUS; DIABETES; GELATINASE; HUMAN;

D O I：

10.3109/02713689408999899

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

The vitreous is a gel-like connective tissue that undergoes liquefaction during aging and pathological processes. We isolated and characterized a degradative enzyme from the vitreous of different species and identified it to be matrix metalloproteinase-2 (MMP-2). This enzyme was found in a latent form. and may be associated with endogenous inhibitors. Vitreous isolated from both non-diabetic and diabetic patients contained MMP-2 in the same concentrations. However, the diabetic samples had an additional gelatinase activity at 92 kDa which may be associated with a compromised vasculature. These results suggest that the normal human vitreous contains an endogenous MMP and the appearance of an additional activity is associated with pathologic conditions.

引用

页码：639 / 647

页数：9

共 35 条

[1]

ALEXANDER JP, 1992, INVEST OPHTHALMOL S, V33, P2137

[2]

BALAZS EA, 1960, J BIOL CHEM, V235, P1973

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] CHARACTERIZATION OF THE MAJOR MATRIX DEGRADING METALLOPROTEINASE OF HUMAN CORNEAL STROMA - EVIDENCE FOR AN ENZYME-INHIBITOR COMPLEX [J].