KERATOCONUS CORNEAS - INCREASED GELATINOLYTIC ACTIVITY APPEARS AFTER MODIFICATION OF INHIBITORS

被引：64

作者：

BROWN, D

CHWA, MM

OPBROEK, A

KENNEY, MC

机构：

[1] Ophthalmology Research Laboratories, Department of Surgery, Cedars-Sinai Research Institute, Cedars-Sinai Medical Center, UCLA Medical School Affiliate, Los Angeles, CA 90048, 8700 Beverly Boulevard, Halper Building

来源：

CURRENT EYE RESEARCH | 1993年 / 12卷 / 06期

关键词：

D O I：

10.3109/02713689309001835

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

We examined the metalloproteinase activity from normal and keratoconus corneal extracts. No differences were detected in the total amount of the metalloproteinase or its physical form of activation. However, there was a significant elevation in enzymatic activity in the keratoconus extracts after chemical modification of inhibitory elements. This suggests either a difference in the enzymatic capabilities of keratoconus corneas or, as suggested previously, a decrease in the amount of TIMP (tissue inhibitor of metalloproteinase) present in the tissue.

引用

页码：571 / 581

页数：11

共 28 条

[1] EVIDENCE FOR THE INVOLVEMENT OF TYPE-II DOMAINS IN COLLAGEN BINDING BY 72-KDA TYPE-IV PROCOLLAGENASE [J].

BANYAI, L ;

PATTHY, L .

FEBS LETTERS, 1991, 282 (01) :23-25

[2] CHARACTERIZATION OF THE MAJOR MATRIX DEGRADING METALLOPROTEINASE OF HUMAN CORNEAL STROMA - EVIDENCE FOR AN ENZYME-INHIBITOR COMPLEX [J].

BROWN, D ;

CHWA, M ;

ESCOBAR, M ;

KENNEY, MC .

EXPERIMENTAL EYE RESEARCH, 1991, 52 (01) :5-16

[3] PURIFICATION OF RABBIT BONE INHIBITOR OF COLLAGENASE [J].

CAWSTON, TE ;

GALLOWAY, WA ;

MERCER, E ;

MURPHY, G ;

REYNOLDS, JJ .

BIOCHEMICAL JOURNAL, 1981, 195 (01) :159-165

[4] GELATINASE AND PROTEOGLYCANASE ACTIVITY DURING THE PERIOVULATORY PERIOD IN THE RAT [J].

CURRY, TE ;

MANN, JS ;

HUANG, MH ;

KEEBLE, SC .

BIOLOGY OF REPRODUCTION, 1992, 46 (02) :256-264

[5] COLLAGENOLYTIC GELATINOLYTIC METALLOPROTEINASES IN NORMAL AND KERATOCONUS CORNEAS [J].

FINI, ME ;

YUE, BYJT ;

SUGAR, J .

CURRENT EYE RESEARCH, 1992, 11 (09) :849-862

[6]

FINI ME, 1990, INVEST OPHTH VIS SCI, V31, P1779

[7] HUMAN 72-KILODALTON TYPE-IV COLLAGENASE FORMS A COMPLEX WITH A TISSUE INHIBITOR OF METALLOPROTEASES DESIGNATED TIMP-2 [J].

GOLDBERG, GI ;

MARMER, BL ;

GRANT, GA ;

EISEN, AZ ;

WILHELM, S ;

HE, CS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (21) :8207-8211

[8] ELECTROPHORETIC ANALYSIS OF PLASMINOGEN ACTIVATORS IN POLYACRYLAMIDE GELS CONTAINING SODIUM DODECYL-SULFATE AND COPOLYMERIZED SUBSTRATES [J].

HEUSSEN, C ;

DOWDLE, EB .

ANALYTICAL BIOCHEMISTRY, 1980, 102 (01) :196-202

[9]

HIBBS MS, 1985, J BIOL CHEM, V260, P2493

[10]

HOWARD EW, 1991, J BIOL CHEM, V266, P13064

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