The amino acid composition of the vascular sap of a high lysine maize mutant was determined during kernel development. With the exception of proline and cystine, all amino acids that occur in the endosperm were found in the vascular sap of the ear peduncle. Glutamine is the major amino acid transported to the endosperm varying from 30.6 to 20.6 μmol at 7 and 42 days after pollination, respectively. Aspartic acid, the second most important nitrogen form translocated to the seeds, was ca 10 μmol% during kernel filling. Glutamine and arginine content decreased with maturity, while valine, methionine, isoleucine, leucine, tyrosine and phenylalanine increased with kernel development. The remaining N forms were constant during endosperm growth. © 1979.