REGENERATION OF PLANTLETS FROM CALLUS-CULTURES OF CHAMAECYTISUS-PURPUREUS AND CHAMAECYTISUS-AUSTRIACUS (LEGUMINOSAE)

Plant regeneration via organogenesis and embryogenesis was obtained from callus cultures of Chamaecytisus purpureus and Chamaecytisus austriacus. While 2,4-D (4 or 0.5 mg/l) proved highly efficient for callus induction, a combination of 0.1 mg/l IBA and 10 mg/l BAP induced a high frequency of shoots from the calli. It is shown that, beside the growth factors, the composition of the basal medium represents a critical factor for regeneration. With increasing culture age, a strong reduction of the differentiation capacity was observed.