REGENERATION OF PLANTLETS FROM CALLUS-CULTURES OF CHAMAECYTISUS-PURPUREUS AND CHAMAECYTISUS-AUSTRIACUS (LEGUMINOSAE)

被引:6
作者
GREINWALD, R
CZYGAN, FC
机构
[1] Institut Für Botanik Und Pharmazeutische Biologie, Universität Würzburg, Würzburg
来源
BOTANICA ACTA | 1991年 / 104卷 / 01期
关键词
CHAMAECYTISUS; PLANT REGENERATION; ORGANOGENESIS; EMBRYOGENESIS;
D O I
10.1111/j.1438-8677.1991.tb00195.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Plant regeneration via organogenesis and embryogenesis was obtained from callus cultures of Chamaecytisus purpureus and Chamaecytisus austriacus. While 2,4-D (4 or 0.5 mg/l) proved highly efficient for callus induction, a combination of 0.1 mg/l IBA and 10 mg/l BAP induced a high frequency of shoots from the calli. It is shown that, beside the growth factors, the composition of the basal medium represents a critical factor for regeneration. With increasing culture age, a strong reduction of the differentiation capacity was observed.
引用
收藏
页码:64 / 67
页数:4
相关论文
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[11]  
WRIGHT MS, 1987, PLANT CELL REP, V6, P83, DOI 10.1007/BF00276659