REVERSIBLE CHANGES IN THE ISOENZYME ELECTROPHORETIC MOBILITY PATTERN AND INFECTIVITY IN CLONES OF TRYPANOSOMA-CRUZI

被引：17

作者：

ALVES, AMB ^{[1
]}

TANURI, A ^{[1
]}

DEALMEIDA, DF ^{[1
]}

VONKRUGER, WMA ^{[1
]}

机构：

[1] UNIV FED RIO DE JANEIRO,INST BIOFIS CARLOS CHAGAS FILHO,FISIOL CELULAR LAB,BR-21949 RIO JANEIRO,BRAZIL

来源：

EXPERIMENTAL PARASITOLOGY | 1993年 / 77卷 / 02期

关键词：

TRYPANOSOMA-CRUZI; ZYMODEME; INFECTIVITY FOR MICE;

D O I：

10.1006/expr.1993.1081

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The stability of zymodemes in clonal cultures of Trypanosoma cruzi derived from strain Y was followed. Reversible changes in the isoenzyme electrophoretic mobility pattern from type A to types B and C were observed after subculturing of cloned cultures in medium of different composition or after passage in newborn mice. Type A zymodeme was observed in clones grown in blood-containing media, while types B and C were found in clones and subclones grown in media progressively less rich in nutrients (10 and 5% fetal calf serum, respectively) and containing no blood. The change in zymodeme from type A to type B or C was associated with loss of infectivity, which could be recovered by passages in newborn mice. Parasites infective for mice always showed zymodeme A. Simultaneously with zymodeme change from type A to types B and C there is a decrease in the specific activity of G6PD and 6PGD. © 1993 Academic Press.

引用

页码：246 / 253

页数：8

共 38 条

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