DNA-LIGASE IS ASSOCIATED WITH THE 21-S-COMPLEX OF ENZYMES FOR DNA-SYNTHESIS IN HELA-CELLS

被引：31

作者：

LI, CG

GOODCHILD, J

BARIL, EF

机构：

[1] WORCESTER FDN EXPTL BIOL INC,SHREWSBURY,MA 01545

[2] HYBRIDON INC,WORCESTER,MA 01605

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 04期

关键词：

D O I：

10.1093/nar/22.4.632

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Approximately 80% of the DNA ligase activity in HeLa cell extracts is associated with the 21 S enzyme complex that functions in simian virus 40 DNA replication in vitro (Malkas at al., Biochemistry 29, 6362 - 6374., 1990). The DNA ligase associated with the 21 S complex was purified extensively and its physical, enzymic and immunological properties characterized as DNA ligase I. The association of DNA ligase I with the 21 S complex of enzymes for DNA synthesis provides evidence for the physiological function; of this DNA ligase in DNA replication in human cells.

引用

页码：632 / 638

页数：7

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