FLUOROMETRIC ESTIMATION OF EPINEPHRINE AND NOREPINEPHRINE - AN IMPROVED MODIFICATION OF TRIHYDROXYINDOLE METHOD

被引：56

作者：

WEILMALHERBE, H

BIGELOW, LB

机构：

来源：

ANALYTICAL BIOCHEMISTRY | 1968年 / 22卷 / 02期

关键词：

D O I：

10.1016/0003-2697(68)90322-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new modification of the trihydroxyindole method for the fluorometric estimation of mixtures of epinephrine and norepinephrine is described. It offers improved sensitivity and discrimination and has a low and stable reagent blank. Its distinctive features are: (1) β-mercaptoethanol is used as reducing agent, (2) the fluorescent solutions are reacidified to approximately pH 5 after alkaline rearrangement to the trihydroxyindole configuration, (3) cupric ions are used as catalysts for the oxidation at pH 3. © 1968.

引用

页码：321 / +

页数：1

共 24 条

[1] ANTON AH, 1962, J PHARMACOL EXP THER, V138, P360
[2] A FLUORIMETRIC METHOD FOR THE DETERMINATION OF DOPAMINE (3-HYDROXYTYRAMINE)
CARLSSON, A
WALDECK, B
[J]. ACTA PHYSIOLOGICA SCANDINAVICA, 1958, 44 (3-4): : 293 - 298
[3] A METHOD FOR THE ESTIMATION OF ADRENALINE AND NORADRENALINE IN URINE
CRAWFORD, TBB
LAW, W
[J]. JOURNAL OF PHARMACY AND PHARMACOLOGY, 1958, 10 (03) : 179 - 188
[4] CROUT J. RICHARD, 1961, STANDARD METHODS CLIN CHEM, V3, P62
[5] EHRLEN I., 1948, FARM REVY, V47, P242
[6] GERST EC, 1966, CLIN CHEM, V12, P659
[7] GLICK D, 1968, METHODS BIOCHEMIC ED, V16
[8] AN IMPROVED METHOD FOR FLUORIMETRIC DETERMINATION OF SMALL AMOUNTS OF ADRENALINE AND NORADRENALINE IN PLASMA AND TISSUES
HAGGENDAL, J
[J]. ACTA PHYSIOLOGICA SCANDINAVICA, 1963, 59 (03): : 242 - &
[9] FLUORIMETRIC DETERMINATION OF 3-O-METHYLATED DERIVATIVES OF ADRENALINE AND NORADRENALINE IN TISSUES AND BODY FLUIDS
HAGGENDAL, J
[J]. ACTA PHYSIOLOGICA SCANDINAVICA, 1962, 56 (3-4): : 258 - +
[10] KAHANE Z, 1965, J LAB CLIN MED, V65, P848

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