FLUOROMETRIC ESTIMATION OF EPINEPHRINE AND NOREPINEPHRINE - AN IMPROVED MODIFICATION OF TRIHYDROXYINDOLE METHOD

被引：56

作者：

WEILMALHERBE, H

BIGELOW, LB

机构：

来源：

ANALYTICAL BIOCHEMISTRY | 1968年 / 22卷 / 02期

关键词：

D O I：

10.1016/0003-2697(68)90322-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new modification of the trihydroxyindole method for the fluorometric estimation of mixtures of epinephrine and norepinephrine is described. It offers improved sensitivity and discrimination and has a low and stable reagent blank. Its distinctive features are: (1) β-mercaptoethanol is used as reducing agent, (2) the fluorescent solutions are reacidified to approximately pH 5 after alkaline rearrangement to the trihydroxyindole configuration, (3) cupric ions are used as catalysts for the oxidation at pH 3. © 1968.

引用

页码：321 / +

页数：1