FLUOROMETRIC ESTIMATION OF EPINEPHRINE AND NOREPINEPHRINE - AN IMPROVED MODIFICATION OF TRIHYDROXYINDOLE METHOD

被引:56
作者
WEILMALHERBE, H
BIGELOW, LB
机构
关键词
D O I
10.1016/0003-2697(68)90322-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A new modification of the trihydroxyindole method for the fluorometric estimation of mixtures of epinephrine and norepinephrine is described. It offers improved sensitivity and discrimination and has a low and stable reagent blank. Its distinctive features are: (1) β-mercaptoethanol is used as reducing agent, (2) the fluorescent solutions are reacidified to approximately pH 5 after alkaline rearrangement to the trihydroxyindole configuration, (3) cupric ions are used as catalysts for the oxidation at pH 3. © 1968.
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页码:321 / +
页数:1
相关论文
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